此電極是設(shè)計用于原位貼壁細(xì)胞的電穿孔或在除樣品杯以外的其他容器內(nèi)進(jìn)行較大量的電穿孔操作，使用電極在原位對貼壁細(xì)胞進(jìn)行電穿孔避免了需使用化學(xué)品消化細(xì)胞以及低穿孔效率，中斷了細(xì)胞周期及細(xì)胞間的聯(lián)系等諸多問題，應(yīng)用于哺乳動物細(xì)胞轉(zhuǎn)染，基因**，蛋白或**的傳遞，也可應(yīng)用于植物和酵母。
此電極是一種可復(fù)用的電極，適用于配裝于6孔板的每個單孔或一個35mm直徑的培養(yǎng)皿，由13個間隔2mm的鍍金電極組成，適用于BTX各型電穿孔儀。
The Petri Pulser™ PP35-2P is a reusable electroporation applicator designed to fit into each single well of a 6-well plate or an individual 35mm diameter petri dish. The PP35-2P consists of an electrode assembly embedded in a polyurethane holder with high voltage electrode cables. The thin electrodes are gold plated and designed to maximize the surface area of electroporation. The high voltage cables are connected with a BTX Pulse Generator, and the electrode head inserted into the dish or well containing the sample. An electroporation pulse may then be delivered. The entire applicator may be cleaned with mild detergent, and the electrodes may be sterilized with ethanol and dried with acetone.
Applications
General Applications
The Petri Pulser PP35-2P is designed for the electroporation of adherent cells in situ or as an alternative to cuvette electroporation for larger volumes or multiple cell samples. The use of the electrode for the electroporation of adherent cells in situ eliminates the need for trypsinization or mechanical removal of cells from their growth substrate. This eliminates associated problems including low plating efficiencies following electroporation, interruption of cell cycle and interruption of intercellular communication.
Mammalian Cell Protein/Drug Electroincorporation
The Petri Pulser PP35-2P supplements our Model 366 Petri Dish Electrode designed for 100 mm petri dishes, as the ideal electrode for in situ electroincorporation of protein into mammalian cells.1,2,3 This electrode has been used for electroincorporation of peptides and proteins in CHO cells4 and vascular smooth muscle cells5.
Mammalian Cell Transfections/Gene Therapy
The Petri Pulser PP35-2P is capable of supporting transfection protocols developed for our larger Model 366 Petri Dish Electrode, including protocols for BHK and HUVEC.6 The electrode itself has been used for the transfection of 3T3-L1, Rat adipose cells, SF295 human neuroglioblastoma and Cos 7 cells.7
Plant, Yeast and Bacterial Electroporation Applications
Applications involving plant, yeast and bacterial electroporation may be carried out using the Petri Pulser. Scale up and multiple sample processing.
Technical Specifications
Standard Capabilities
Voltage Range: 0 - 300V
Pulse Length Range: 1 - 35 msec in PBS
Volume Range: 0.5 - 3.0 ml
Physical Characteristics
Weight: 6 oz
Number of Electrodes: 13
Electrode Thickness: 0.5 mm
Electrode Material: Gold Plated.
Generator Compatibility ECM 830, 630, 600, 399, 395, T 820
Ordering Information
System Model Number Available Configurations
PP35-2P Petri Pulser PP35-2P and Instruction Sheet
References
Marrero et al., Journal of Biological Chemistry, 270 (26): 15734-15738 (1995)
Scheiffer et al., Journal of Biological Chemistry, 271 (17): 10329-10333 (1996)
Marrero et al., Journal of Biological Chemistry, 272 (39): 24684-24690 (1997)
Lobo, Errol, University of California, San Francisco, Personal Communication (1998)
Fukai, Masuko, Emory University, Personal Communication (1998)
BTX Protocols PR0165 and PR0330 (1998)
BTX Protocols PR0290, PR00286, PR0359, PR0360, PR0364 (1998)