微波固定系統(tǒng)
（實驗動物腦內(nèi)酵素瞬間滅活性系統(tǒng)）
Muromachi Microwave Fixation system
原理與特色：
1. 用微波加熱的方式使得神經(jīng)組織內(nèi)的酶（ENZYME）去活性，以避免死后變化
（post-mortem changes）持續(xù)進展。
2. 此法比用冷凍固定法要來得好：冷凍法需要10-90秒做深入冷凍去活性，這段
期間，深部組織的死后變化（post-mortem changes）已經(jīng)在進行，因腦表面
的熱傳導轉差。微波法可在更短的時間內(nèi)完全去活性。
3. 可以測：
* Acetylcholine乙酰膽堿
* Choline 膽堿
* Cyclic AMP 環(huán)腺苷酸
* Cyclic GMP 環(huán)林鳥苷
* GABA 伽馬氨基丁酸
* DOPA 多巴（二羥基苯丙氨酸）
* 5-HTP 5-羥色胺酸
* Serotonin 血清素
* Endorphin 內(nèi)啡肽
* Prostaglandin 前列腺素
* Catecholamines and their metabolites 兒茶酚胺及其代謝物
* Phosphorylated Proteins 磷酸化蛋白質(zhì)
微波固化可以達到下列標準：
1. 可將溫度升至75-90攝氏度，使腦部快速固定。
2. 微波能量可有效集中在動物的頭部。
3. 各種動物間都可以達到相同的結果。
4. 對操作人員具備嚴格的保護，此設備相當簡單**
規(guī)格
振蕩器
Toshiba Magnetron 2M68
微波輸出
5kW max.
震蕩頻率
2.450±30MHz
冷卻要求
水冷
水流量
每分鐘兩升
暴露時間
0.01-9.99秒 (0.01秒步進)
暫停時間
3 分鐘
能量功耗
9.7千瓦
頻率
50 或者 60 Hz
電源要求
200/220VAC 40A 3Phase
外形尺寸
935W x 640D x 1,310H 厘米
重量
大約 220公斤
|
APPLICATOR HEAD |
適用的動物管 |
| TAW-174A | WJM-24 for Mouse 15-20g WJM-28 for Mouse 20-40g |
| TAW-424SA | WJR-S for Rat 150-250g |
| TAW-424M | WJR-M for Rat 250-400g WJR-L for Rat 400-500g |
水套式動物管（左側的是大鼠固定器，右側的是老鼠固定器）
|
標準系統(tǒng)包括： | |
| Main Unit TMW-4012C | 1 |
| Applicator Head ( Any one from the list ) | 1 |
| Animal Holder ( Any one from the list ) | 1 |
| Dust Cover | 1 |
Unambiguous assignment of the H3S and H3R deuterations of cerebral (2-13C) glutamate by 13C NMR at 18.8 tesla
TB Rodrigues, IR Violante, S … - Magnetic Resonance in …, 2010 - interscience.wiley.com
... At the end of the infusions, cerebral metabolism was arrested using a 5- kW microwave
fixation system, the brain being rapidly removed from the skull and immediately frozen in liquid N2. ...
Brain glutamine synthesis requires neuronal-born aspartate as amino donor for glial glutamate formation
... regulated circulating water bath. Fifteen minutes after 13 C-label administration, the
metabolism was arrested using a high-power (5 kW) focused microwave fixation system
The brain was ...
Delayed Increase of Brain Noradrenaline After Acute Footshock Stress in Rats
... 4.5–5 kW, 1.4 s) using a small animal microwave applicator...
Immediately after microwave radiation, the brains were harvested and put onto an ice-chilled
Petri dish ... Moroji T, Takahashi T, Ogura K, Toishi T, Arai S (1977) Rapid fixation of rat ...
Altered Affect, Monoamine Transmitters and Bioenergetic Homeostasis of Alpha-synuclein-transgenic Mice, in the Presence and Absence of Endogenous Alpha- EM Cumyn - 2010 - tspace.library.utoronto.ca
... 36 3.7 Open field test..... 37 3.8 Microwave fixation and dissection of brain tissue.....
37 3.9 Fresh dissection and flash-freezing of brain tissue..... 40 ...
Brainstem Concentrations of Cholesterol are not Influenced by Genetic Ablation of the Low-Density Lipoprotein Receptor
AY Taha, CT Chen, Z Liu, JH Kim, HTJ Mount, … - Neurochemical …, 2009 - Springer
... At 7 weeks of age, the mice were euthanized with a high-energy head-focused microwave
fixation system (6 kW, 0.88–0.99 s, Muromachi brain fixation system in order to stop brain lipid metabolism [14, 15]. ...
Detection of reduced GABA synthesis following inhibition of GABA transaminase using in vivo magnetic resonance signal of [13C] GABA C1
J Yang, C Johnson, J Shen - Journal of neuroscience methods, 2009 - Elsevier
... After in vivo MRS data acquisition of gabaculine-treated rats infused with [2,5- 13 C 2 ]glucose
and unlabeled glucose, the rats were euthanized using a microwave fixation device (Model
TNW-6402C, 5 kW, 1.5 s). Subsequently, the rat ...
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Brainstem Concentrations of Cholesterol are not Influenced by Genetic Ablation of the Low-Density Lipoprotein Receptor
Detection of reduced GABA synthesis following inhibition of GABA transaminase using in vivo magnetic resonance signal of [13C] GABA C1